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trxA [2019-10-30 11:19:40]
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trxA [2019-10-30 11:19:40]

thioredoxin, antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange, important for the prevention of protein aggregation during severe heat stress
locus
BSU_28500
pI
4.31
mw
11.25 kDa
protein length
104 aa Sequence Blast
gene length
315 bp Sequence Blast
function
protection of proteins against oxidative damage
product
thioredoxin
essential
yes
synonyms
trx
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,913,024 2,913,338

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Protein family

  • [SW|Thioredoxin family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|A245594D3F37ACE6B99855DDDFEC27F7F21579E4|YdbP], [protein|4A8B6D88FEA9D18E1BAE08847A5F7683F9D6CDF4|YosR]

    • [SW|Domains]

  • Thioredoxin domain (aa 2-104) (according to UniProt)

    • Structure

  • [PDB|2GZZ] (oxidized form), [PDB|2GZY] (reduced form), [PDB|2IPA] (TrxA-ArsC-complex) [Pubmed|17303556]

    • [SW|Localization]

  • cytoplasm [Pubmed|17501922]

    • Expression and Regulation

    Operons

    genes
    [gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]
    description
    [Pubmed|9537387]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9537387], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9537387,11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|29271514], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • induced by heat stress ([protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]) [Pubmed|9852015]

    • additional information

  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|29271514]
  • A [protein|search|ncRNA] is predicted between [protein|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|TrxA] and [protein|1DF08A474E95909EAF68CF26D97DD49350ABEA89|Abf2] [PubMed|20525796]
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    Biological materials

    Expression vector

  • pGP2327: for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 23899494

    • Original publications

  • 9537387,15937154,18601268,18456801,9852015,11544224,18687074,20084284,17303556,12642660,20525796,17501922,24401092,24417481,29271514,30855939